BEAM Lab - Peptide Unit
Group Leader : Alessandro Gori
02 285 00039 | alessandro.gori@icrm.it
Istituto
Istituto di Chimica del Riconoscimento Molecolare
Via Mario Bianco 9, 20131 Milano
Tel. +39 02 285.000.20
http://www.icrm.cnr.it/
Membri del Laboratorio
Monica Sani, Renato Longhi, Zana Pina
Attività
- Peptide science
- Synthesis of peptide epitopes for vaccination purposes
- Synthesis of peptide bioprobes for immunoassays
- Structural engineering of peptide epitopes/ peptide bioprobes
- Bioconjugation strategies for peptide epitopes/ peptide bioprobes
Strumenti / Tecnologie
- Microwave-assisted solid phase peptide synthesis
- Analytical and semipreparative RP-HPLC
Genome Human Department
Group Leader : Anna Villa
0282245160 – 0226435273 | anna.villa@humanitasresearch.it
Istituto
Istituto di Ricerca Genetica e Biomedica
UOS Milano via Fantoli 15/16 – 20090 Milano (MI)
Tel. +39 0282245158
https://www.irgb.cnr.it
Membri del Laboratorio
Barbara Cassani, Veronica Marrella, Rosita Rigoni
Attività
Immunology- autoimmunity- Primary Immunodeficienccy- gene therapy in mouse models and Humans.
Strumenti / Tecnologie
Lentiviral transduction , gene therapy
Human Genome
Group Leader : Barbara Cassani
02/82245153 | barbara.cassani@humanitasresearch.it
Istituto
IRGB UOS Milan (c/o Istituto Clinico Humanitas)
Via Manzoni 113, 20089 Rozzano (MI)
https://www.irgb.cnr.it/
Attività
Our major lines of research are:
1) Host-microbiota interactions in the pathogenesis of immune-mediated diseases
2) Redox signalling in the regulation of intestinal homeostasis and cancer
3) Dissecting the interplay between adaptive immune responses and cellular senescence in tumorigenesis
Principali progetti e collaborazioni
We have projects founded by the Italian Ministry of Health. We are collaborating with major research hospitals in Milan.
Strumenti / Tecnologie
Deep multiparameter FACS Analysis, Histology, NSG Sequencing, metabolomics, gut organoids, mouse models of disease, cancer and infections.
Chromatin and Nuclear Architecture
Group Leader : Chiara Lanzuolo
lanzuolo@cnr.it – 3297429357
Istituto
Istituto di Tecnologie Biomediche (ITB) and Istituto Nazionale Genetica Molecolare (INGM)
Via Francesco Sforza 35, Milano
www.itb.cnr.it | www.ingm.cnr.it
Membri del Laboratorio
Federica Lucini, PhD student Giulia La Macchia, undergraduate student
Attività
My group is devoted in understanding how the genome folding occur in the nuclear space finding the right orientation and nuclear position and how this conformation is then maintained or regulated in dynamic physiological processes in health and in disease. Studying human primary CD4+ T lymphocytes we found an unconventional genomic organization where the heterochromatin is susceptible to DNAse digestion while the euchromatin is insoluble. This CD4+ T cell-specific chromatin conformation clearly distinguish lymphocytes from any other cell population analysed, opening unprecedented perspectives on the relation between chromatin organization and function.
Strumenti / Tecnologie
Genome wide analysis with a new technology developed in our laboratory: the SAMMYseq: https://www.biorxiv.org/content/10.1101/799668v1
Altre Informazioni
Skills in epigenetics and chromatin higher order structures
Cell-to-cell communication in brain
Group Leader | Claudia Verderio
0250317011| c.verderio@in.cnr.it
Istituto
Istituto di Neuroscienze
Via Vanvitelli 32 20129 Milano
Tel. 02 50316964
http://www.ic.cnr.it
Membri del Laboratorio
Ilaria Prada, Martina Gabrielli, Giulia D’Arrigo, Marta Lombard
Attività
- Microglia-neuron, microglia-OPC crosstalk via extracellular vesicles
Strumenti / Tecnologie
- Calcium imaging, confocal live imaging combined to optical manipulation
Biocomputing pEptide Analytical Microsystems (BEAM) Lab Biocomputing Group
Group Leader : Giorgio Colombo
02-28500031
g.colombo@icrm.cnr.it
Istituto
Istituto di Chimica del Riconoscimento Molecolare
Via Mario Bianco, 9 – 20131- Milano
Tel. +39 02 285.000.20
http://www.icrm.cnr.it
Membri del Laboratorio
Permanent Staff: Massimiliano Meli, Giulia Morra
7 Post-docs and one Undergrad student
Attività
The Biocomputing pEptide Analytical Microsystems (BEAM) Lab at ICRM-CNR is the result of a shared vision between three distinct research teams within the institute operating in the field of immunology at different levels. Aiming to predict the antibody-binding regions of a protein, the Biocomputing group at ICRM have developed a new approach based on the integrated analysis of the dynamical and energetic properties of antigens, to identify non-optimized, low-intensity energetic interaction networks in the protein structure isolated in solution. The method is based on the idea that recognition sites may correspond to localized regions with low-intensity energetic couplings with the rest of the protein, which allows them to undergo conformational changes, to be recognized by a binding partner, and to tolerate mutations with minimal energetic expense. We are currently exploring possible implications of the methodology in vaccine design and in the targeting of protein-protein interactions.
Insights gained from these methods are used in our lab to design peptidic epitope mimics that could be used to elicit antibodies able to recognize the original full-length proteins and/or to be used as new diagnostic probes. Peptides are in fact powerful and versatile tools for diagnostic applications; for example, epitope collections can be exploited to unreveal the amount of immunological information contained in serum, profiling personalized immuno-response to infection, vaccination, allergens and autoimmunity and providing hints on new biomarkers for cancer or neurodegenerative diseases.
Strumenti / Tecnologie
- High Performance Computing CPU Cluster
- High Performance Computing GPU Cluster
- 15 high end workstations, Full licenses for Discovery Studio, MAESTRO, ICM. Access to GROMACS, AMBER16, CHARMM through academic licenses
More information at: http://beamlabicrm.weebly.com
NMR structural biology
Group Leader : Laura Ragona
+39-02-23699619 (office) | laura.ragona@ismac.cnr.it
Istituto
Istituto per lo Studio delle Macromolecole
Via Corti 12, 20133 Milano
Tel. +39 2 23699351/353
http://www.ismac.cnr.it
Membri del Laboratorio
Simona Tomaselli (ric III°) simona.tomaselli@ismac.cnr.it
Katiuscia Pagano katiuscia.pagano@ismac.cnr.it
Henriette Molinari (research associate) henriette.molinari@univr.it
Attività
- Structure, dynamics and interaction studies of proteins, small molecules, lipids
- Role of protein aggregation in neurodegenerative diseases
- NMR based design of antiangiogenic and antitumoral drugs, targeting fibroblast growth factor (FGF) interactions
- Lipid carriers (intracellular lipid binding proteins
- Ferritin
- Development and molecular characterization of functional biomaterials
Strumenti / Tecnologie
- Nuclear Magnetic Resonance facility (400 MHz, 500 MHz, 600 MHz) for structural analysis of samples of biological interest
- Computational facility for data driven docking and protein structure determination
Biocomputing pEptide Analytical Microsystems (BEAM) Lab - Micro-analytical Division
Group Leader – Marina Cretich
marina.cretich@icrm.cnr.it | 39-0228500042
Istituto
Istituto di Chimica del Riconoscimento Molecolre
Via Mario Bianco, 9
20131 Milan, Italy
Tel. +39 02 285.000.20
www.icrm.cnr.it
Membri del Laboratorio
- Marcella Chiari
- Laura Sola
- Francesco Damin
Attività
The Biocomputing pEptide Analytical Microsystems (BEAM) Lab at ICRM-CNR is the result of a shared vision between three distinct research teams within the institute operating in the field of immunology at different levels. The micro-analytical division develops bio-chemical tools and strategies to deliver new antibody/protein/peptide based bioassays for circulating protein biomarkers and to advance immunodiagnostics. The lab has an internationally recognized expertise in polymer chemistry, surface modification of miniaturized analytical systems and nanoparticles, DNA, protein and peptide microarrays, development of innovative bioassays, capillary and microchip electrophoresis. The group produces high-sensitivity fluorescence microarrays and also applies its methods to diverse analytical platforms, from ELISA and SPR to rapid tests, focusing on many clinical needs including exosome analysis by EV (Extracellular vesicles) arrays; infectious diseases, inflammation, autoimmunity and allergy. The group has a consolidated know-how on antibody screening and validation of clinical tests
Strumenti / Tecnologie
Complete platform for in-house development and production of fluorescence microarrays (piezoelectric spotter Scienion S12, two laser scanners, incubation stations, interferometric set up for quantification of immobilized probes). Instrumentation for slab gel, capillary and microchip electrophoresis, basic equipment for molecular biology and protein biochemistry. Nanosight – NTA (Nanoparticle Tracking Analysis) System
More information at:
https://microlabicrm.weebly.com
Pharmacology and Brain Pathology
Group Leader : Michela Matteoli
02 82245202 / 02 50317097
m.matteoli@in.cnr.it
Istituto
Istituto di Neuroscienze
Via Vanvitelli 32, 20129 Milano
Tel. 02 50316964
http://www.ic.cnr.it
Membri del Laboratorio
CNR Researchers: Elisabetta Menna and Maria Luisa Malosio
Humanitas RTD: Davide Pozzi
Humanitas Staff Scientist: Raffaella Morini
Postdocs: Fabia Filipello, Lorena Passoni, Irene Corradini, Eliana Lauranzano, Matteo Tamborini, Romana Tomasoni, Eleonora Vannini, Chiara Elia, Marco Rasile, Patricia Correia
PhD students: Alice Canzi, Elisa Focchi, Filippo Mirabella, Genni Desiato, Cristina Mantovani
Attività
- Cellular and molecular basis of synapse function and plasticity;
- role of the immune system in synapse formation; cell biology of microglia;
- neurodevelopmental diseases (autism and intellectual disability);
- neurodegenerative diseases (Alzheimer’s disease).
Strumenti / Tecnologie
- Primary cultures of neurons, astrocytes and microglia;
- basic biochemical and molecular biology techniques;
- electrophysiology in slices and cultures;
- single cell imaging (and calcium imaging);
- in vitro and ex vivo confocal microscopy, STED microscopy, two-photon microscopy, FRAP, FRET;
- mouse behavioural analysis;
- institutional FACS facility.